![]() To more objectively evaluate the in vivo distribution and clearance of tracers, the pharmacokinetics and biodistribution assays were also performed. The clinical application value of I-HPP-durva-F(ab') 2 was confirmed by PET imaging. After the radiochemical purity, stability, and specific activities were determined, the binding affinities of probes prepared using different labeling strategies were compared in vitro. The prepared durva F(ab') 2 fragments were incubated with N-succinimidyl-3-(4-hydroxyphenyl) propionate (SHPP) after purification, the HPP-durva F(ab') 2 was iodinated using Iodo-Gen method. ![]() The aim of this study was to explore an effective 124I labeling strategy and improve the signal-to-noise ratio when evaluating the expression of PD-L1 using an 124I-iodinated durvalumab (durva) F(ab') 2 fragment.
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